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lonA [2019-09-20 09:49:07]
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lonA [2019-09-20 09:49:07]

class III heat-shock ATP-dependent protease
locus
BSU_28200
pI
5.77
mw
86.42 kDa
protein length
774 aa Sequence Blast
gene length
2325 bp Sequence Blast
function
protein quality control, control of swarming motility
product
class III heat-shock ATP-dependent protease
essential
no
synonyms
lon
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]

    • Gene

    Coordinates
    2,880,466 → 2,882,790

    Phenotypes of a mutant

  • presence of predifferentiated swarmer cells in liquid medium [Pubmed|25538299]

    • The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of proteins in presence of ATP (according to UniProt)
  • inhibits swarming motility by preventing accumulation of SwrA in liquid medium [Pubmed|25538299]

    • Protein family

  • Peptidase S16 family (with [protein|1A5C1BDE844AA56B90C0E4A02D74978676487E99|DdcP] and [protein|CA90AAE5320C20F93F5ED0F167294C28495756FF|LonB], according to UniProt)

    • Structure

  • [PDB|5E7S] (active hexamer from ''Meiothermus taiwanensis'', 63% identity, 89% similarity) [Pubmed|27041593]
  • [PDB|4YPN] (ADP-bound hexamer from ''Meiothermus taiwanensis'', 63% identity, 89% similarity) [Pubmed|27041592]
  • [PDB|3M65] (N-terminal domain) [Pubmed|20600124]
  • [PDB|3M6A] (C-terminal domain) [Pubmed|20600124]
  • [PDB|1X37] (Ssd domain)

    • [SW|Localization]

  • coincident with the nucleoid during normal growth and localized to the forespore during development [Pubmed|18689473]

    • Expression and Regulation

    Operons

    genes
    [gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]-[gene|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC]
    description
    [Pubmed|7961402]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7961402], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9852015], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]

    • regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]
    • view in new tab

    Biological materials

    Mutant

  • BKE28200 (Δ[gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACTGACACCTCCGT, downstream forward: _UP4_AAATGAAAGTCACAAAGTCA
  • BKK28200 (Δ[gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACTGACACCTCCGT, downstream forward: _UP4_AAATGAAAGTCACAAAGTCA

    • References

    Reviews

  • 23479438,26639779

    • Original publications

  • 7961403,7961402,19542270,19643080,9852015,18689473,20600124,25538299,27041592,27041593,29311275